Incucyte® Nuclight Red Jurkat Cells are supplied as 1 mL cryopreserved vials (1.5 x 106 cells/mL in 90% FBS and 10% DMSO) containing a stable population of human T lymphocyte cells expressing the Incucyte® Nuclight Red fluorescent protein, restricted to the nucleus. Parental Jurkat (clone E6.1) cells (ATCC, Cat. No. TIB-152) were transduced with the Incucyte® Nuclight Red Lentivirus (Cat. No. 4476; EF1α, puromycin). 48 hours after transduction, the cell population was grown in complete growth media containing 1 µg/ml puromycin, for 3 to 5 days, to select for cells expressing Incucyte® Nuclight Red. Following selection, Incucyte® Nuclight Red Jurkats were validated by comparing morphology and chemotactic migration profiles to the parental cell line. The Incucyte® Nuclight Red Jurkats are certified mycoplasma free and are fully validated for use with the Incucyte® Live-Cell Analysis System. These cells are ideal for use in real-time cell counting studies, co-cultures and chemotaxis assays.