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Incucyte® Neuroprime Orange Kit

Incucyte® Neuroprime Orange Kit

A complete solution for performing kinetic, live-cell imaging of 96-well co-culture assays utilizing primary rat cortical neurons and astrocytes. Supplied as 1 vial (0.45 mL) Incucyte® Neurolight Orange Lentivirus (Synapsin promoter), 1 vial (2 x 106 cells) cryopreserved Incucyte® rCortical Neurons, 1 vial (2 x 106 cells) cryopreserved Incucyte® rAstrocytes.
(Cat. No. 4760)

Neuroprime Orange Kit Contents

The kit is intended for use on Incucyte® Live-Cell Analysis Systems configured with a Green/Orange/NIR or an Orange/NIR Optical Module, along with the Incucyte® Neurotrack Analysis Software Module (Cat. No. 9600-0010).

Key Features

  • Cryopreserved vials of cortical neurons and astrocytes isolated from early embryonic stage (E18) Sprague-Dawley rats (1x vial of each, 2 x 106 cells/vial)
  • Incucyte® Neurolight Orange Lentivirus, expressing the orange fluorescent protein TagRFP under control of a Synapsin promoter (1x vial, 0.45 mL)
  • Minimal expression of fluorescence in non-neuronal cell types
  • The Neurolight Orange Lentivirus is non-perturbing to neuronal biology
  • Validated protocol for optimal neuron labeling and assay performance
  • Ideal for studies of neurotoxicity or general biology of established neural networks

Example Images and Segmentation

Neurite outgrowth of primary rat cortical neurons in co-culture with rat astrocytes measured using the Neuroprime Orange Kit and Neurotrack Analysis Software Module. Upper left: Primary rat cortical neurons in co-culture were labeled with the Neurolight Orange Lentivirus and imaged seven days post plating at 20x. Upper right: Corresponding HD-phase contrast image. Lower left: Automated image segmentation of neurites (turquoise). Lower right: Automated image segmentation of cell body clusters (purple).

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