Incucyte® NucLight Green Lentivirus Reagent (EF-1 Alpha Promoter, Puromycin selection) - 0.6mL
One vial of Lentivirus reagent (0.6 mL; >1x10^6 TU/mL) suitable for transducing cells with a non-perturbing nuclear restricted red fluorescent label. Stable cell lines can be created under puromycin selection.
Incucyte® NucLight Lentivirus Reagents enable efficient, non-perturbing, nuclear labelling of living mammalian cells. They are compatible with convenient transduction protocols and enable real-time cell counting and the calculation of cell doubling times. Incucyte NucLight Lentivirus Reagents provide homogenous expression of a nuclear-restricted GFP (green fluorescent protein) or mKate2 (red fluorescent protein) in your choice of primary, immortalized, dividing, or non-dividing cells without altering cell function and with minimal toxicity. These reagents are ideal for generating stable cell populations or clones using puromycin or bleomycin selection. The Incucyte NucLight Lentivirus Reagents have been validated for use with the Incucyte® live-cell analysis system and can be combined with the Incucyte® Caspase-3/7 Reagent, Incucyte® Annexin V Reagents or Incucyte® Cytotox Reagents for multiplexed measurements of apoptosis and cytotoxicity alongside cell proliferation in a single well.
Virus Description: 3rd generation HIV-based, VSV-G pseudotyped lentiviral particles encoding a nuclear restricted GFP. Promoter: EF-1 alpha. Selectable Marker: Puromycin. Spectral Properties: Ex (max): 483 nm; Em (max): 506 nm