Overview of the Incucyte® Clearview Plate design
The Incucyte® Clearview Plate for chemotaxis assays combines the throughput of transmembrane assays with the optical clarity of microfluidics devices. Each well of a Incucyte® Clearview Plate provides an optically clear surface for label-free imaging and analysis of chemotactic cell migration using the Incucyte® Live-Cell Analysis System.
The Clearview membrane contains 96 laser etched pores with a diameter of 8µm. Cells are added to the upper chamber and chemoattractant to the lower reservoir plate. Chemotactic transmembrane migration is visually monitored within your incubator and automatically analyzed using the Incucyte® Live-Cell Analysis System.
Each Incucyte® Clearview Plate is composed of:
- One plate lid (A)
- One 96-well Clearview membrane insert (B)
- One 96-well reservoir plate (C)
Visualize every cell
- The specially designed Clearview membrane enables you to monitor every cell throughout your experiment in real time – gain deep phenotypic insight into cell movement and morphology
Monitor every cell in your experiment. The Clearview membrane enables label-free visualization and quantitation of chemotaxis using Incucyte® Live-Cell Analysis System. Each membrane contains 96 precision engineered holes in a regular grid array. Three pores are highlighted in yellow.
Low cell usage ideal for rare, expensive and primary cell populations
- Use 10- to 100-fold fewer cells than standard transmembrane (e.g. Boyden Chamber) approaches
Lower cell usage. Cell usage comparison for the Incucyte® Clearview Plate and Corning® Transwell® system. Jurkat T cell chemotaxis toward SDFα-1 was measured in both systems (2,000 to 20,000 cells/well); however, no signal was observed with the Transwell plate. A minimum of 150,000 cells/well was required to observe chemotactic migration using the Corning Transwell system.
Biologically relevant surface-mediated migration
- Cells must migrate across a biologically relevant surface towards the chemoattractant due to the low pore density of the specially designed Clearview membrane (pore density <2% that of Boyden chamber transwells)
- The Clearview plate has been validated with a wide range of biomatrix coatings, cell types and chemoattractants
Cells must interact with substrate to reach a pore. Time-lapse images of a Jurkat T cell (outlined in white) migrating across a Matrigel® coating towards a pore leading to the chemoattractant SDF-1α (orange circle). White arrows indicate the direction of Jurkat movement in each frame. In the final image the Jurkat T cell has entered the pore and moved to the lower chamber.
Stable and sustained gradients
- Chemotactic gradients are stable and maintained over 72 hours
Long-term stable gradients compared to traditional Boyden chamber transwells. (A) Gradient stability was monitored over 72 hours using an Alexa Fluor® 594 dextran (10 kDa). After 4 hours a rapid loss in gradient was measured in the Corning Transwell system. In contrast, >80% of the gradient was maintained in the Incucyte® Clearview Plate at 72 hours. (B) FBS gradients pre-established in a Incucyte® Clearview Plate for 24, 48, and 72 hours induced equivalent rates of directional migration in HT-1080 cells indicating long-term and stable gradients across the Clearview membrane. Rates were slightly faster than for cells migrating towards gradients that had not been pre-established. This is likely a result of increased cell health when serum starved cells are plated in established gradients.
Compatible with standard automation and liquid dispensing devices
- 96-well format suitable for screening workflows
- Compliant with SBS microtiter plate requirements
Comparison of the Incucyte® Clearview Plate with existing Boyden chamber transwell and microfluidic approaches
|Clearview||Boyden Chambers||Microfluidic Devices|
|Visually observe cell
movement and morphology
|Stable chemotactic gradient|
|Low cell usage (< 5,000 per well)|
|Surface interactions– Integrin sensitivity|
|96-well throughput or higher|
|Workflow: set up and walk away|
|Product Name||Qty||Catalog No.|
|Incucyte® Clearview 96-Well Chemotaxis Plate||1 plate||4582|
|Incucyte® Clearview 96-Well Chemotaxis Plates – Case of 10 Plates||10 plates||4648|
|Incucyte® Clearview 96-Well Chemotaxis Plates – Case of 50 Plates||50 plates||4599|
|Incucyte® Clearview Reservoir Plate – Case of 10 Plates||10 plates||4600|
|Incucyte® Clearview Reservoir Plate – Case of 100 Plates||100 plates||4601|