The Incucyte® pHrodo® Red Cell Labeling Kit enables the labeling of your choice of target cells with a pH-sensitive fluorophore for use in IncuCyte® phagocytosis assays. Each kit includes:
- One vial of IncuCyte® pHrodo® Red Cell Labeling dye
- One vial of DMSO used to solubilize the dye
- One bottle of wash buffer
- One bottle of labeling buffer
The unique pHrodo®-based cell labeling system exploits the acidic environment of the phagosome to quantify phagocytosis. As Incucyte® pHrodo® Red labeled cells residing in the neutral extracellular solution (pH 7.4) are engulfed by phagocytes and enter the acidic phagosome (pH 4.5 – 5.5), a substantial increase in fluorescence is observed. In the absence of phagocytes, the fluorescence intensity of the labeled cells remains low. The Incucyte® pHrodo® Red Cell Labeling Kit has been validated for use with the Incucyte® Live-Cell Analysis System configured with a Green/Red Optical Module and enables real-time evaluation of phagocytic regulation by pharmacological agents as well as genetic and environmental factors.